Ajiboye Basiru Olaitan, Oyinloye Babatunji Emmanuel, Ojo Oluwafemi Adeleke, Lawal Olaolu Ebenezer, Jokomba Yesirat Abimbola, Balogun Basheer Ajibola, Adeoye Akinwunmi Oluwaseun, Ajuwon Olawale Rasaq
Phytomedicine and Molecular Toxicology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria.
Institute of Drug Research and Development, SE Bogoro Center, Afe Babalola University, Ado-Ekiti, Nigeria.
Bioinform Biol Insights. 2022 Aug 10;16:11779322221115546. doi: 10.1177/11779322221115546. eCollection 2022.
The antioxidant, cholinergic, monoaminergic, and purinergic activities of flavonoid-rich extract from leaf (FEDW) were investigated on oxidative testicular injury (ex vivo) due to the local report on the use of this plant as anti-testicular injury. Flavonoid extract was obtained from FEDW using a standard procedure. Five male albino rats were used, testes harvested and incubated with FeSO for accessing the cholinergic, monoaminergic, and purinergic activities of the FEDW (ex vivo). Testicular tissues incubated with FeSO demonstrated a significant decrease in antioxidant biomarkers, arginase, ATPase, ENTPDase, 5'-nucleotidase, and PDE-5 activities, as well as Zn and sialic acid levels with an upsurge in malondialdehyde (MDA), and NO levels, myeloperoxidase, cholinesterases, monoamine oxidase (MAO), and angiotensin-converting enzyme (ACE) activities. Treatment of testicular tissues incubated with FeSO via different concentrations of FEDW significantly increased the activities of antioxidant, arginase, ATPase, E-NTPDase, 5'-nucleotidase, phosphodiesterase-5 (PDE-5), as well as Zn and sialic acid levels with a significant decrease in MDA, nitric oxide (NO), myeloperoxidase, cholinesterases, MAO, and ACE levels. Molecular docking revealed the molecular interactions of cyclooxygenase-2 (COX-2) with ellagic acid, piperine, and caffeine with piperine and caffeine obeyed the druggability and pharmacokinetic. These findings point to FEDW as a possible potential for the treatment of oxidative testicular injury.
由于当地有关于该植物用于抗睾丸损伤的报道，因此对叶中富含黄酮类化合物的提取物（FEDW）的抗氧化、胆碱能、单胺能和嘌呤能活性进行了体外氧化睾丸损伤研究。采用标准程序从FEDW中获得黄酮类提取物。使用5只雄性白化病大鼠，取出睾丸并与硫酸亚铁一起孵育，以评估FEDW的胆碱能、单胺能和嘌呤能活性（体外）。用硫酸亚铁孵育的睾丸组织显示抗氧化生物标志物、精氨酸酶、ATP酶、ENTPD酶、5'-核苷酸酶和PDE-5活性以及锌和唾液酸水平显著降低，同时丙二醛（MDA）、一氧化氮（NO）水平、髓过氧化物酶、胆碱酯酶、单胺氧化酶（MAO）和血管紧张素转换酶（ACE）活性升高。用不同浓度的FEDW处理与硫酸亚铁孵育的睾丸组织，可显著提高抗氧化、精氨酸酶、ATP酶、E-NTPD酶、5'-核苷酸酶、磷酸二酯酶-5（PDE-5）的活性以及锌和唾液酸水平，同时MDA、一氧化氮（NO）、髓过氧化物酶、胆碱酯酶、MAO和ACE水平显著降低。分子对接揭示了环氧化酶-2（COX-2）与鞣花酸、胡椒碱和咖啡因的分子相互作用，其中胡椒碱和咖啡因符合可药用性和药代动力学。这些发现表明FEDW可能具有治疗氧化睾丸损伤的潜力。
