Brouazin-Jousseaume Valérie, Guitton Nathalie, Legué Florence, Chenal Christian
Centre Eugène Marquis, Equipe de Radiobiologie, UPRES EA No223 1, Rennes, France.
Anticancer Res. 2002 Jan-Feb;22(1A):257-62.
The overproduction of ROS by ionizing irradiation induces cellular damage which can be reduced by specific molecules such as GSH and cytokines. The aim of this study was to determine the relationship between the well known radioresistance of Sertoli cells and astrocytes in vitro and the GSH level and IL-6 production after irradiation.
Cell viability, GSH content and IL-6 production were assessed at different times after irradiation and for different doses, on rat Sertoli cells and astrocytes.
After irradiation we observed a dose-dependent increase in the intracellular total GSH level and IL-6 production as compared to the controls.
These results strongly suggest the key role of GSH and IL-6 in the mechanisms of response of radioresistant cells to gamma irradiation. One hypothesis is that the increase of GSH level and IL-6 production after irradiation contributes to the adaptative response to oxidative stress generated by gamma irradiation.
电离辐射导致的活性氧（ROS）过量产生会引起细胞损伤，而谷胱甘肽（GSH）和细胞因子等特定分子可减轻这种损伤。本研究的目的是确定体外已知的支持细胞和星形胶质细胞的放射抗性与照射后GSH水平和白细胞介素-6（IL-6）产生之间的关系。
在照射后的不同时间以及不同剂量下，对大鼠支持细胞和星形胶质细胞的细胞活力、GSH含量和IL-6产生进行评估。
与对照组相比，照射后我们观察到细胞内总GSH水平和IL-6产生呈剂量依赖性增加。
这些结果强烈表明GSH和IL-6在放射抗性细胞对γ射线照射的反应机制中起关键作用。一种假设是照射后GSH水平和IL-6产生的增加有助于对γ射线照射产生的氧化应激的适应性反应。
