Ochi Y, Hachiya T, Yoshimura M, Miyazaki T, Majima T
Endocrinol Jpn. 1975 Aug;22(4):351-6. doi: 10.1507/endocrj1954.22.351.
A specific double antibody radioimmunoassay has been develop for the measurement of thyroglobulin in human serum. Human thyroglobulin was purified by combined DEAE-cellulose and affinity chromatography using Sepharose 4B-bound Concanavalin A. Sensitivity of test serum was 10 ng/ml. Thyroglobulin was not detectable in half of normal subjects, and half showed values between 10 and 180 ng/ml. In the patients with simple goiter and secondary hypothyroidism, serum thyroglobulin was usually in the normal range. In Hashimoto's thyroiditis, many sera having precipitating antibodies or high hemagglutination antibodies for thyroglobulin showed a high thyroglobulin concentration in serum probably due to a false positive reaction. In hyperthyroidism, an increased thyroglobulin level was observed in 64% of patients. However, there was no correlation between serum thyroglobulin and thyroxine levels in untreated hyperthyroidism. Serum thyroglobulin was increased significantly in some cases for several weeks after isotope therapy for the hyperthyroidism.
已开发出一种特异性双抗体放射免疫测定法，用于测定人血清中的甲状腺球蛋白。人甲状腺球蛋白通过结合DEAE - 纤维素和使用琼脂糖4B结合的伴刀豆球蛋白A的亲和色谱法进行纯化。检测血清的灵敏度为10 ng/ml。一半的正常受试者中检测不到甲状腺球蛋白，另一半的甲状腺球蛋白值在10至180 ng/ml之间。在单纯性甲状腺肿和继发性甲状腺功能减退患者中，血清甲状腺球蛋白通常在正常范围内。在桥本甲状腺炎中，许多对甲状腺球蛋白有沉淀抗体或高血凝抗体的血清显示血清中甲状腺球蛋白浓度较高，这可能是由于假阳性反应所致。在甲状腺功能亢进症患者中，64%的患者甲状腺球蛋白水平升高。然而，未经治疗的甲状腺功能亢进症患者血清甲状腺球蛋白与甲状腺素水平之间没有相关性。在对甲状腺功能亢进症进行同位素治疗后的几周内，某些病例的血清甲状腺球蛋白显著升高。
