Pezzino V, Cozzani P, Filetti S, Galbiati A, Lisi E, Squatrito S, Vigneri R
Eur J Clin Invest. 1977 Dec;7(6):503-8. doi: 10.1111/j.1365-2362.1977.tb01643.x.
A specific double-antibody radioimmunoassay with a sensitivity of 2.5 ng/ml has been developed for measuring thyroglobulin (Tg) in human serum. As endogenous anti-Tg antibodies in serum interfere in the assay, only sera with a negative tanned red cell (TRC) test are suitable for analysis. Tg was detectable in 84.7% of the euthyroid subjects, with a mean value of 6.1 (values ranging from nondetectable to 43.0 ng/ml). Values were significantly higher in women than in men. Tg release by the thyroid appears to be under pituitary control, as suggested by TSH stimulation and T3 suppression tests. Elevated Tg levels were found in hyperthyroidism, simple goitre, and differentiated thyroid carcinoma. The significance of circulating Tg and the possible application of the Tg RIA are discussed.
已开发出一种灵敏度为2.5 ng/ml的特异性双抗体放射免疫测定法，用于检测人血清中的甲状腺球蛋白（Tg）。由于血清中的内源性抗Tg抗体干扰该测定，只有鞣酸红细胞（TRC）试验呈阴性的血清才适合进行分析。84.7%的甲状腺功能正常受试者可检测到Tg，平均值为6.1（值范围为不可检测到43.0 ng/ml）。女性的值显著高于男性。促甲状腺激素刺激试验和T3抑制试验表明，甲状腺释放Tg似乎受垂体控制。在甲状腺功能亢进症、单纯性甲状腺肿和分化型甲状腺癌中发现Tg水平升高。讨论了循环Tg的意义以及Tg放射免疫测定法的可能应用。
