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优化氨处理条件以增强豆科植物对纤维水解的敏感性:佛罗里达草甸根茎花生

Optimizing ammonia processing conditions to enhance susceptibility of legumes to fiber hydrolysis: Florigraze rhizoma peanut.

作者信息

Ferrer A, Byers F M, Sulbarán-de-Ferrer B, Dale B E, Aiello C

机构信息

Department of Chemistry, Science Faculty, University of Zulia, Maracaibo, Venezuela.

出版信息

Appl Biochem Biotechnol. 2002 Spring;98-100:135-46. doi: 10.1385/abab:98-100:1-9:135.

DOI:10.1385/abab:98-100:1-9:135
PMID:12018243
Abstract

A warm-season legume, Florigraze rhizoma peanut (FRP), was used as the source of fiber to produce sugars. FRP was subjected to several ammonia-processing conditions using temperature, biomass moisture content, and ammonia loading as process variables during a 5-min treatment. A cellulase loading of 2 FPU/g DM and 24 h incubation were used to produce the sugars. Total sugar yield was 3.34-fold higher in the optimal treatment (1.5 g ammonia/g DM-60%-90 degrees C) compared to untreated and was 65.3% of theoretical. Cellulose and hemicellulose conversions increased from 30 and 15.5% in untreated FRP to 78 and 34% in treated FRP.

摘要

一种暖季型豆科植物——弗洛里格拉兹根茎花生(FRP),被用作生产糖的纤维来源。在5分钟的处理过程中,以温度、生物质含水量和氨负载量作为工艺变量,对FRP进行了几种氨处理条件的实验。使用2 FPU/g DM的纤维素酶负载量和24小时的孵育时间来生产糖。与未处理的相比,最佳处理(1.5 g氨/g DM - 60% - 90摄氏度)的总糖产量高出3.34倍,为理论值的65.3%。纤维素和半纤维素的转化率从未处理的FRP中的30%和15.5%分别提高到处理后的FRP中的78%和34%。

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