School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Bioresour Technol. 2013 Jun;138:314-20. doi: 10.1016/j.biortech.2013.03.091. Epub 2013 Mar 21.
A novel lignocellulose pretreatment method using aqueous ammonia for biofuel production was proposed in this study, which named recycled aqueous ammonia expansion (RAAE). Effects of temperature, pretreatment time, water to dry corn stalks loading and flow rate of aqueous ammonia on substrate enzymatic digestibility and sugar yield were investigated. Pretreatment temperature and time are important factors that affect RAAE process. Recirculation process could improve biomass digestibility and sugar yield compared with batch experiment. After RAAE pretreatment, about 70% of the lignin was removed, while more than 90% of the cellulose was preserved in the solids, the substrate crystallinity also increased because of the removal of amorphous portion. The maximum glucan enzymatic digestibility of pretreated biomass was 85.70%, which was obtained at 85°C, 11 min, 80% water to dry corn stalks loading and 1.5L/min aqueous ammonia flow rate.
本研究提出了一种利用氨水溶液进行木质纤维素预处理以生产生物燃料的新方法，称为循环氨膨胀（RAAE）。考察了温度、预处理时间、水对干玉米秸秆的装载量和氨水溶液流速对基质酶解和糖产量的影响。预处理温度和时间是影响 RAAE 过程的重要因素。与分批实验相比，循环过程可以提高生物质的消化率和糖产量。RAAE 预处理后，约 70%的木质素被去除，而超过 90%的纤维素保留在固体中，由于无定形部分的去除，基质结晶度也增加。预处理生物质的最大葡聚糖酶解率为 85.70%，在 85°C、11 分钟、80%水对干玉米秸秆装载量和 1.5L/min 氨水溶液流速下获得。
