Karmarkar S H
Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, Thrissur, India.
Indian J Exp Biol. 2001 Dec;39(12):1263-7.
Hairy roots were induced from shoot buds and seedling hypocotyls of Holostemma by infection with agropine type Agrobacterium rhizogenes strains. Type of explant, Agrobacterium rhizogenes strain used for infection, co-culture time and photoperiod influenced the transformation frequencies. Hairy roots were induced from seedling hypocotyls and shoot bud explants upon infection with agropine type Agrobacterium rhizogenes strains. The hairy roots were thin, whitish in colour and showed negatively geotropic growth. The transformed nature of hairy roots was confirmed by opine analysis.
通过用章鱼碱型发根农杆菌菌株感染，从全冠藤的茎芽和幼苗下胚轴诱导出毛状根。外植体类型、用于感染的发根农杆菌菌株、共培养时间和光周期影响转化频率。在用章鱼碱型发根农杆菌菌株感染后，从幼苗下胚轴和茎芽外植体诱导出毛状根。毛状根细，呈白色，表现出负向地性生长。通过冠瘿碱分析证实了毛状根的转化性质。
