[Genetic transformation of hairy roots in Trichosanthes kirilowii Maxim. by Ti and Ri plasmids].

OBJECTIVE

To establish a hairy root culture system by double transformation for Trichosanthes kirilowii.

METHOD

1. Crown galls were induced by direct infection of sterile seedlings of T. kirilonii with Agrobacterium tumefaciens C58, and then the hairy roots were obtained from the regenerated plants by infection with A. rhzogenes 15834; 2. Transformation of Ti and Ri plasmids was inspected by high-pressure-paper electrophoresis; 3. The protein contents in the tissues of T. kirilowii were inspected by spectrophotometer and SDS-PAGE.

RESULT

A hairy root culture system has been established successfully by double transformation with Ti and Ri plasmids in T. kirilowii.

CONCLUSION

Compared with the ordinary hairy roots, the double transformed hairy roots grow faster but retain similar protein contents.