Bortkiewicz Alicja, Pilacik Bozena, Gadzicka Elzbieta, Szymczak Wieslaw
Nofer Institute of Occupational Medicine, Lodz, Poland.
Neuro Endocrinol Lett. 2002 Apr;23 Suppl 1:88-91.
It is quite likely that non-visible electromagnetic fields (EMF) may affect melatonin production. Some studies confirmed this hypothesis and showed that extremely low EMF altered pineal function in animals and humans. Thus, it is reasonable to suppose that EMF emitted by cellular phones may also influence secretion of melatonin. The present study sought to evaluate possible effect of the exposure to EMF emitted by cellular phone on 6-hydroxymelatonin sulfate (6-OHMS) excretion, which reflects melatonin levels in blood.
The examined group consisted of 9 healthy males aged 19-29 years. The experiment was performed under controlled conditions (the light intensity-50 lx till midnight and 0 lx during night). Each person was examined twice: on a day without exposure (control day, C-day) and on a day with continuous exposure (60 min. exposure from cellular phone, frequency 900 MHz, pulsed with 217 Hz, pulse with 576 micros, SAR 1.23 W/kg, E-day). From 7 p.m. to 8 p.m. they used a cellular phone. The subjects did not know which day was E-day, and which was C-day. From 8 p.m. till midnight the subjects listened to music and than they slept till 7 a.m. next day. Urine samples were collected at 7 p.m., at midnight, and at 7 a.m. in the same way in C-day as in E-day. Sample were frozen for later ELISA analysis of 6-OHMS. The 6-OHMS ELISA kit from Immuno-Biological Laboratories (Hamburg) was used for measurement of 6-OHMS. The data were analysed using Wilcoxon matched-pairs signed-ranks test for each subject and for the whole group. We compared 6-OHMS level on the E-day and on the C-day separately for 3 time-points - 7 p.m., midnight, 7 a.m.
Mean 6-OHMS level in both experiments did not differ significantly for any of the respective time points. Circadian variations of 6-OHMS level were detected in all subjects.
The results of our investigation has demonstrated that EMF emitted by cellular phones has no distinct influence on the melatonin level.
不可见的电磁场（EMF）很可能会影响褪黑素的分泌。一些研究证实了这一假设，并表明极低频率的电磁场会改变动物和人类的松果体功能。因此，有理由推测手机发出的电磁场也可能影响褪黑素的分泌。本研究旨在评估暴露于手机发出的电磁场对硫酸6-羟基褪黑素（6-OHMS）排泄的可能影响，6-OHMS排泄反映了血液中的褪黑素水平。
受试组由9名年龄在19至29岁之间的健康男性组成。实验在可控条件下进行（光照强度——午夜前为50勒克斯，夜间为0勒克斯）。每个人接受两次检测：一次是在无暴露日（对照日，C日），另一次是在持续暴露日（使用手机暴露60分钟，频率900兆赫，脉冲频率217赫兹，脉冲宽度576微秒，比吸收率1.23瓦/千克，E日）。从晚上7点到8点他们使用手机。受试者不知道哪一天是E日，哪一天是C日。从晚上8点到午夜，受试者听音乐，然后一直睡到第二天早上7点。在C日和E日，均于晚上7点、午夜和早上7点以相同方式收集尿液样本。样本冷冻保存，以备后续进行6-OHMS的酶联免疫吸附测定（ELISA）分析。使用免疫生物实验室（汉堡）的6-OHMS ELISA试剂盒测量6-OHMS。对每个受试者和整个组的数据使用威尔科克森配对符号秩检验进行分析。我们分别在3个时间点——晚上7点、午夜、早上7点比较了E日和C日的6-OHMS水平。
在任何相应时间点，两个实验中的6-OHMS平均水平均无显著差异。在所有受试者中均检测到6-OHMS水平的昼夜变化。
我们的调查结果表明，手机发出的电磁场对褪黑素水平没有明显影响。
