Koehler J K
J Cell Biol. 1975 Dec;67(3):647-59. doi: 10.1083/jcb.67.3.647.
Antibodies to rabbit spermatozoa isolated from immunized virgin female rabbits were conjugated to hemocyanin and have been used to determine the pattern of antigenic sites over the surface of rabbit spermatozoa under a variety of conditions. When the spermatozoa are incubated with antibody at 0 degrees C, labeling is almost entirely restricted to the postacrosomal sheath. Incubation at room temperature (22 degrees C) or at 37 degrees C results in progressively increased acrosomal labeling as well as enhanced postacrosomal sheath labeling. Initial incubation at low temperature followed by washing and high temperature incubation yields cells which indicate little tendency for postacrosomal sheath sites to migrate toward the acrosomal region. Absorption of the antibody preparation with rat lymphocytes has no effect on labeling density, whereas absorption with rabbit lymphocytes diminishes the intensity of labeling and yields cells with markers primarily over the postacrosomal sheath. It is concluded that the postacrosomal sheath plasma membrane has specific antigenic properties distinct from other regions of the head.
从免疫过的未交配雌兔中分离出的抗兔精子抗体与血蓝蛋白偶联，并已用于在各种条件下确定兔精子表面抗原位点的模式。当精子在0℃下与抗体孵育时，标记几乎完全局限于顶体后鞘。在室温（22℃）或37℃下孵育会导致顶体标记逐渐增加以及顶体后鞘标记增强。先在低温下进行初始孵育，然后洗涤并在高温下孵育，得到的细胞显示顶体后鞘位点几乎没有向顶体区域迁移的趋势。用大鼠淋巴细胞吸收抗体制剂对标记密度没有影响，而用兔淋巴细胞吸收则会降低标记强度，并产生主要在顶体后鞘上带有标记的细胞。得出的结论是，顶体后鞘质膜具有与头部其他区域不同的特定抗原特性。
