Qu-Petersen Zhuqing, Deasy Bridget, Jankowski Ron, Ikezawa Makato, Cummins James, Pruchnic Ryan, Mytinger John, Cao Baohong, Gates Charley, Wernig Anton, Huard Johnny
Growth and Development Laboratory, Children's Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh, PA 15260, USA.
J Cell Biol. 2002 May 27;157(5):851-64. doi: 10.1083/jcb.200108150. Epub 2002 May 20.
Three populations of myogenic cells were isolated from normal mouse skeletal muscle based on their adhesion characteristics and proliferation behaviors. Although two of these populations displayed satellite cell characteristics, a third population of long-time proliferating cells expressing hematopoietic stem cell markers was also identified. This third population comprises cells that retain their phenotype for more than 30 passages with normal karyotype and can differentiate into muscle, neural, and endothelial lineages both in vitro and in vivo. In contrast to the other two populations of myogenic cells, the transplantation of the long-time proliferating cells improved the efficiency of muscle regeneration and dystrophin delivery to dystrophic muscle. The long-time proliferating cells' ability to proliferate in vivo for an extended period of time, combined with their strong capacity for self-renewal, their multipotent differentiation, and their immune-privileged behavior, reveals, at least in part, the basis for the improvement of cell transplantation. Our results suggest that this novel population of muscle-derived stem cells will significantly improve muscle cell-mediated therapies.
根据其黏附特性和增殖行为，从正常小鼠骨骼肌中分离出三类生肌细胞。尽管其中两类细胞表现出卫星细胞特征，但还鉴定出了第三类表达造血干细胞标志物的长期增殖细胞。这第三类细胞包括那些在核型正常的情况下传代超过30次仍保持其表型的细胞，并且在体外和体内均可分化为肌肉、神经和内皮谱系。与其他两类生肌细胞不同，长期增殖细胞的移植提高了肌肉再生效率以及向营养不良肌肉递送抗肌萎缩蛋白的效率。长期增殖细胞在体内长时间增殖的能力，再加上其强大的自我更新能力、多能分化能力以及免疫豁免行为，至少部分揭示了细胞移植改善的基础。我们的结果表明，这种新型的肌肉来源干细胞群体将显著改善肌肉细胞介导的治疗。
