Puigderrajols Pere, Jofré Anna, Mir Gisela, Pla Maria, Verdaguer Dolors, Huguet Gemma, Molinas Marisa
Laboratori del Suro, Facultat de Ciències, Universitat de Girona, 17071 Girona, Spain.
J Exp Bot. 2002 Jun;53(373):1445-52.
The timing and tissue localization of small heat shock proteins (sHSPs) during cork oak somatic embryo development was investigated under normal growing culture conditions and in response to stress. Western blot analyses using polyclonal antibodies raised against cork oak recombinant HSP17 showed a transient accumulation of class I sHSPs during somatic embryo maturation and germination. Moreover, the amount of protein increased at all stages of embryo development in response to exogenous stress. The developmentally accumulated proteins localized to early differentiating, but not the highly dividing, regions of the root and shoot apical meristems. By contrast, these highly dividing regions were strongly immunostained after heat stress. Findings support the hypothesis of a distinct control for developmentally and stress-induced accumulation of class I sHSPs. The possible role of sHSPs is discussed in relation to their tissue specific localization.
在正常生长培养条件下以及响应胁迫时，研究了栓皮栎体细胞胚胎发育过程中小热激蛋白（sHSPs）的时间和组织定位。使用针对栓皮栎重组HSP17产生的多克隆抗体进行的蛋白质印迹分析表明，I类sHSPs在体细胞胚胎成熟和萌发过程中短暂积累。此外，响应外源胁迫时，胚胎发育各阶段的蛋白质含量均增加。发育积累的蛋白质定位于根和茎顶端分生组织早期分化而非高度分裂的区域。相比之下，热胁迫后这些高度分裂的区域被强烈免疫染色。研究结果支持了对I类sHSPs发育性和胁迫诱导积累进行不同控制的假设。结合sHSPs的组织特异性定位讨论了其可能的作用。
