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揭示 早期体细胞胚胎发生过程中活跃的激素转录调控现象。

Uncovering a Phenomenon of Active Hormone Transcriptional Regulation during Early Somatic Embryogenesis in .

机构信息

School of Grassland Science, Beijing Forestry University, Beijing 100083, China.

出版信息

Int J Mol Sci. 2022 Aug 3;23(15):8633. doi: 10.3390/ijms23158633.

DOI:10.3390/ijms23158633
PMID:35955760
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9368939/
Abstract

Somatic embryogenesis (SE) is a developmental process in which somatic cells undergo dedifferentiation to become plant stem cells, and redifferentiation to become a whole embryo. SE is a prerequisite for molecular breeding and is an excellent platform to study cell development in the majority of plant species. However, the molecular mechanism involved in somatic embryonic induction, embryonic and maturation is unclear. This study was designed to examine the differentially expressed genes (DEGs) and miRNA roles during somatic embryonic induction, embryonic and maturation. The cut cotyledon (ICE), non-embryogenic callus (NEC), embryogenic callus (EC) and cotyledon embryo (CE) were selected for transcriptome and small RNA sequencing. The results showed that 17,251 DEGs, and 177 known and 110 novel miRNAs families were involved in embryonic induction (ICE to NEC), embryonic (NEC to EC), and maturation (EC to CE). Expression patterns and functional classification analysis showed several novel genes and miRNAs involved in SE. Moreover, embryonic induction is an active process of molecular regulation, and hormonal signal transduction related to pathways involved in the whole SE. Finally, a miRNA-target interaction network was proposed during SE. This study provides novel perspectives to comprehend the molecular mechanisms in SE.

摘要

体细胞胚胎发生（SE）是体细胞经历去分化成为植物干细胞，再经过再分化成为整个胚胎的发育过程。SE 是分子育种的前提，是研究大多数植物物种细胞发育的极好平台。然而，体细胞胚胎诱导、胚胎发生和成熟过程中涉及的分子机制尚不清楚。本研究旨在研究体细胞胚胎诱导、胚胎发生和成熟过程中的差异表达基因（DEGs）和 miRNA 作用。选择离体子叶（ICE）、非胚胎性愈伤组织（NEC）、胚胎性愈伤组织（EC）和子叶胚（CE）进行转录组和小 RNA 测序。结果表明，17251 个 DEGs 和 177 个已知和 110 个新的 miRNA 家族参与了胚胎诱导（ICE 到 NEC）、胚胎发生（NEC 到 EC）和成熟（EC 到 CE）。表达模式和功能分类分析显示，SE 涉及一些新的基因和 miRNA。此外，胚胎诱导是一个分子调控的活跃过程，与整个 SE 相关的激素信号转导途径有关。最后，提出了 SE 过程中的 miRNA-靶相互作用网络。本研究为理解 SE 中的分子机制提供了新的视角。

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