猫杯状病毒：转染cRNA或cDNA构建体后野生型和重组病毒的恢复
Feline calicivirus: recovery of wild-type and recombinant viruses after transfection of cRNA or cDNA constructs.
作者信息
Thumfart Jörg Oliver, Meyers Gregor
机构信息
Institute of Immunology, Federal Research Centre for Virus Diseases of Animals, D-72001 Tübingen, Germany.
出版信息
J Virol. 2002 Jun;76(12):6398-407. doi: 10.1128/jvi.76.12.6398-6407.2002.
Abstract
The RNA genome of the vaccine strain 2024 of feline calicivirus was cloned as cDNA and analyzed by nucleotide sequencing. A full-length DNA copy of the viral genome was established and proved to be a source of infectious cRNA after in vitro transcription and RNA transfection. Virus could also be recovered when the DNA construct was introduced into cells containing phage T7 RNA polymerase that was provided by vaccinia virus MVA-T7. After insertion of the sequence encoding the green fluorescent protein into the structural protein-encoding region of the infectious cDNA clone, a defective replicon was recovered that was able to replicate autonomously and was packaged into virus particles when the structural proteins were provided in trans.
摘要
猫杯状病毒疫苗株2024的RNA基因组被克隆为cDNA，并通过核苷酸测序进行分析。建立了病毒基因组的全长DNA拷贝，并证明其在体外转录和RNA转染后是感染性cRNA的来源。当将该DNA构建体引入含有由痘苗病毒MVA-T7提供的噬菌体T7 RNA聚合酶的细胞中时，也能回收病毒。将编码绿色荧光蛋白的序列插入感染性cDNA克隆的结构蛋白编码区后，获得了一种缺陷复制子，该复制子能够自主复制，并且在反式提供结构蛋白时被包装成病毒颗粒。
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