Rostoks Nils, Park Yong-Jin, Ramakrishna Wusirika, Ma Jianxin, Druka Arnis, Shiloff Bryan A, SanMiguel Phillip J, Jiang Zeyu, Brueggeman Robert, Sandhu Devinder, Gill Kulvinder, Bennetzen Jeffrey L, Kleinhofs Andris
Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164, USA.
Funct Integr Genomics. 2002 May;2(1-2):51-9. doi: 10.1007/s10142-002-0055-5. Epub 2002 Apr 25.
Barley (Hordeum vulgare L.) is one of the most important large-genome cereals with extensive genetic resources available in the public sector. Studies of genome organization in barley have been limited primarily to genetic markers and sparse sequence data. Here we report sequence analysis of 417.5 kb DNA from four BAC clones from different genomic locations. Sequences were analyzed with respect to gene content, the arrangement of repetitive sequences and the relationship of gene density to recombination frequencies. Gene densities ranged from 1 gene per 12 kb to 1 gene per 103 kb with an average of 1 gene per 21 kb. In general, genes were organized into islands separated by large blocks of nested retrotransposons. Single genes in apparent isolation were also found. Genes occupied 11% of the total sequence, LTR retrotransposons and other repeated elements accounted for 51.9% and the remaining 37.1% could not be annotated.
大麦（Hordeum vulgare L.）是最重要的大基因组谷物之一，公共部门拥有丰富的遗传资源。对大麦基因组组织的研究主要限于遗传标记和稀疏的序列数据。在此，我们报告了来自不同基因组位置的四个BAC克隆的417.5 kb DNA的序列分析。对序列进行了基因含量、重复序列排列以及基因密度与重组频率关系方面的分析。基因密度范围从每12 kb有1个基因到每103 kb有1个基因，平均每21 kb有1个基因。一般来说，基因被组织成由大片嵌套反转录转座子分隔的岛状结构。也发现了明显孤立的单个基因。基因占总序列的11%，LTR反转录转座子和其他重复元件占51.9%，其余37.1%无法注释。
