豇豆蚜传花叶病毒的基因组序列及其与其他马铃薯Y病毒属病毒的相似性。
The genomic sequence of cowpea aphid-borne mosaic virus and its similarities with other potyviruses.
作者信息
Mlotshwa S, Verver J, Sithole-Niang I, Van Kampen T, Van Kammen A, Wellink J
机构信息
Laboratory of Molecular Biology, Wageningen University, The Netherlands.
出版信息
Arch Virol. 2002 May;147(5):1043-52. doi: 10.1007/s00705-002-0800-z.
The genomic sequence of a Zimbabwe isolate of Cowpea aphid-borne mosaic virus (CABMV-Z) was determined by sequencing overlapping viral cDNA clones generated by RT-PCR using degenerate and/or specific primers. The sequence is 9465 nucleotides in length excluding the 3' terminal poly (A) tail and contains a single open reading frame (ORF) of 9159 nucleotides encoding a large polyprotein of 3,053 amino acids and predicted Mr of 348. The size of the genome and the encoded polyprotein is in agreement with other potyviruses and contains nine putative proteolytic cleavage sites and motifs conserved in homologous proteins of other potyviruses. The P1 and P3 were the most variable proteins while CI, NIb and CP were the most conserved.
通过使用简并引物和/或特异性引物对经逆转录聚合酶链反应（RT-PCR）产生的重叠病毒互补DNA（cDNA）克隆进行测序，确定了来自津巴布韦的豇豆蚜传花叶病毒分离株（CABMV-Z）的基因组序列。该序列长度为9465个核苷酸，不包括3'末端的聚腺苷酸（poly (A)）尾，包含一个9159个核苷酸的单一开放阅读框（ORF），编码一个由3053个氨基酸组成的大的多聚蛋白，预测分子量为348。基因组大小和编码的多聚蛋白与其他马铃薯Y病毒一致，并且包含九个推定的蛋白水解切割位点以及在其他马铃薯Y病毒的同源蛋白中保守的基序。P1和P3是最可变的蛋白，而CI、NIb和CP是最保守的。
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