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比较分析两个不同宿主来源的豇豆花叶病毒(CABMV)分离株的基因组。

Comparative analysis of the genomes of two isolates of cowpea aphid-borne mosaic virus (CABMV) obtained from different hosts.

机构信息

Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.

出版信息

Arch Virol. 2011 Jun;156(6):1085-91. doi: 10.1007/s00705-011-0962-7. Epub 2011 Mar 16.

Abstract

The complete genomic sequences of two cowpea aphid-borne mosaic virus (CABMV) isolates from Brazil, MG-Avr from passion fruit (which also infects cowpea), and BR1 from peanut (which also infects cowpea, but not passion fruit), were determined. Their nucleotide sequences are 89% identical and display 85% identity to that of CABMV-Z. Both isolates have the typical potyvirus genome features. P3 and VPg are the most conserved proteins, with 99% amino acid sequence identity between the two isolates, and P1 is the most variable, with 50% identity. A significant variation exists at the 5'-end of the genome between the Brazilian isolates and CABMV-Z. However, this variation does not correlate with the biological properties of these three isolates.

摘要

从巴西的两种豇豆花叶病毒(CABMV)分离物中,MG-Avr 来自西番莲果(也感染豇豆),BR1 来自花生(也感染豇豆,但不感染西番莲果),确定了它们的完整基因组序列。它们的核苷酸序列同源性为 89%,与 CABMV-Z 的同源性为 85%。这两种分离物均具有典型的马铃薯 Y 病毒科基因组特征。P3 和 VPg 是最保守的蛋白质,两个分离物之间的氨基酸序列同一性为 99%,而 P1 是最可变的,同一性为 50%。巴西分离物与 CABMV-Z 之间在基因组的 5'端存在显著的变异。然而,这种变异与这三种分离物的生物学特性无关。

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