利用深度测序技术测定的澳大利亚西番莲木质部病毒分离株的全基因组序列及其与其他马铃薯 Y 病毒科病毒的关系。
The complete genome sequence of a Passion fruit woodiness virus isolate from Australia determined using deep sequencing, and its relationship to other potyviruses.
机构信息
Plant Virus Section, Western Australian State Agricultural Biotechnology Centre, Murdoch University, Perth, WA 6150, Australia.
出版信息
Arch Virol. 2011 Mar;156(3):479-82. doi: 10.1007/s00705-010-0845-3. Epub 2010 Nov 13.
The complete genome sequence (9,858 nucleotides) of the Passion fruit woodiness virus isolate MU-2 was determined using Illumina sequencing. The large open reading frame (ORF) encodes a polyprotein containing 3,086 amino acids, with an AUG start codon and UAA stop codon. The polyprotein yielded 11 proteins (P1, HC-Pro, P3, PIPO, 6K1, CI, 6K2, NIa-VPg, NIa-Pro, NIb and CP). Putative cleavage sites between them were identified by sequence comparison to those of other known potyviruses. Accuracy of the genome sequence information was provided by 42-1691-fold sequence coverage, and viral RNA accounted for 7.38% of total polyadenylated RNA from the host plant.
使用 Illumina 测序技术，确定了 MU-2 分离物的西番莲木质化病毒的完整基因组序列（9858 个核苷酸）。 该大开放阅读框（ORF）编码一个包含 3086 个氨基酸的多聚蛋白，具有 AUG 起始密码子和 UAA 终止密码子。 多聚蛋白产生 11 种蛋白（P1、HC-Pro、P3、PIPO、6K1、CI、6K2、NIa-VPg、NIa-Pro、NIb 和 CP）。 通过与其他已知的马铃薯 Y 病毒科病毒的序列比较，鉴定了它们之间的推定切割位点。 通过 42-1691 倍序列覆盖度提供了基因组序列信息的准确性，并且病毒 RNA 占宿主植物总多聚腺苷酸化 RNA 的 7.38％。
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