Suh Young Suk, Oda Sen ich, Kang Yoon Hee, Kim Hyun, Rhyu Im Joo
Department of Anatomy, College of Medicine and Human Genetics, Institute of Medicine Science Research Center, Korea University, 126-1 Anam-Dong 5-Ga, Sungbuk-Ku, Seoul 136-705, South Korea.
Neurosci Lett. 2002 May 31;325(1):1-4. doi: 10.1016/s0304-3940(02)00216-1.
Rolling mouse Nagoya is a voltage dependent calcium channel alpha1A subunit mutant showing moderate ataxia. Granule cell loss was previously reported in the cerebellar vermis of homozygous rolling. Apoptotic cerebellar granule cell death was reported in homozygous leaner mice, an allele of rolling. Cerebella of 21-day-old rolling and wild type mice were used for terminal dUTP nick-end-labeling (TUNEL) assay and electron microscopic observation to understand the mechanism of granule cell loss in rolling mice. The number of TUNEL-positive cells was significantly increased in rolling. More TUNEL-positive cells were observed in the anterior cerebellar vermis compared with posterior. Condensation and fragmentation of granule cell nuclei in rolling mouse were observed frequently. These results suggest that apoptosis is one of the mechanisms of granule cell loss in the rolling cerebellum.
滚动小鼠名古屋品系是一种表现出中度共济失调的电压依赖性钙通道α1A亚基突变体。此前报道，纯合滚动小鼠的小脑蚓部存在颗粒细胞丢失。滚动小鼠的一个等位基因——纯合倾斜小鼠，曾被报道出现凋亡性小脑颗粒细胞死亡。本研究使用21日龄滚动小鼠和野生型小鼠的小脑进行末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）检测和电子显微镜观察，以了解滚动小鼠颗粒细胞丢失的机制。滚动小鼠中TUNEL阳性细胞数量显著增加。与后部相比，在前部小脑蚓部观察到更多TUNEL阳性细胞。在滚动小鼠中经常观察到颗粒细胞核的凝聚和碎片化。这些结果表明，凋亡是滚动小鼠小脑中颗粒细胞丢失的机制之一。
