Rhyu I J, Oda S, Uhm C S, Kim H, Suh Y S, Abbott L C
Institute of Human Genetics and Department of Anatomy, Korea University College of Medicine, Seoul, South Korea.
Neurosci Lett. 1999 Apr 30;266(1):49-52. doi: 10.1016/s0304-3940(99)00254-2.
Rolling mouse Nagoya (rolling: tg(rol)) is a neurologic mutant mouse exhibiting severe ataxia. Two alleles of the rolling mutation, tottering (tg) and leaner(tg(la)), have been identified as mutations in the voltage-dependent calcium channel alpha1A subunit. No specific light and electron microscopic findings have been reported for the rolling mouse cerebellum except a decreased number of granule cells, while altered Purkinje cell/parallel fiber synapses have been observed in tottering and leaner cerebella. Rolling mouse cerebella were analyzed using anti-calbindin-D immunohistochemistry and transmission electron microscopy to investigate Purkinje cell morphology and synaptic contacts between Purkinje cell dendritic spines and parallel fiber varicosities. Multiple Purkinje cell dendritic spines synapsing with single parallel fiber varicosities were frequently observed in rolling cerebella. The correlation between the presence of altered Purkinje cell synapses and ataxia in rolling mice warrants further investigation.
滚动小鼠名古屋品系（滚动：tg(rol)）是一种表现出严重共济失调的神经突变小鼠。滚动突变的两个等位基因，蹒跚（tg）和更瘦（tg(la)），已被鉴定为电压依赖性钙通道α1A亚基的突变。除了颗粒细胞数量减少外，滚动小鼠小脑尚无具体的光镜和电镜观察结果报道，而在蹒跚和更瘦小鼠的小脑中观察到浦肯野细胞/平行纤维突触改变。使用抗钙结合蛋白-D免疫组织化学和透射电子显微镜对滚动小鼠小脑进行分析，以研究浦肯野细胞形态以及浦肯野细胞树突棘与平行纤维膨体之间的突触联系。在滚动小鼠小脑中经常观察到多个浦肯野细胞树突棘与单个平行纤维膨体形成突触。滚动小鼠中浦肯野细胞突触改变与共济失调之间的相关性值得进一步研究。
