Wang Lixin, Saint-Pierre David H, Taché Yvette
CURE: Digestive Diseases Research Center, VA Greater Los Angeles Healthcare System, Department of Medicine, University of California at Los Angeles, Building 115, Room 203, 11301 Wilshire Boulevard, Los Angeles, CA 90073, USA.
Neurosci Lett. 2002 May 31;325(1):47-51. doi: 10.1016/s0304-3940(02)00241-0.
Ghrelin, a peptide isolated from the rat stomach, is the endogenous ligand of the growth hormone-secretagogue receptor and also known to have orexigenic effect. We examined the influence of intraperitoneal (i.p.) injection of ghrelin on food intake and brain neuronal activity in freely fed mice. Ghrelin (3, 10 or 30 microg/mouse) dose-dependently increased food intake by 0.8-, 1.6- and 2.6-fold, respectively, at 30 min post injection. Ghrelin (10 microg/mouse) induces Fos expression selectively in the ventromedial part of the hypothalamic arcuate nucleus (Arc). No change in Fos expression was observed in other hypothalamic and hindbrain nuclei. About 90% of the Fos-positive neurons in the Arc expressed neuropeptide Y (NPY) messenger RNA. These data indicate that NPY neurons in the Arc are likely the primary target mediating i.p. ghrelin induced orexigenic effect.
胃饥饿素是一种从大鼠胃中分离出的肽，是生长激素促分泌素受体的内源性配体，也已知具有促食欲作用。我们研究了腹腔注射胃饥饿素对自由进食小鼠食物摄入量和脑神经元活动的影响。注射胃饥饿素（3、10或30微克/只小鼠）后30分钟，食物摄入量分别以剂量依赖方式增加了0.8倍、1.6倍和2.6倍。胃饥饿素（10微克/只小鼠）选择性地诱导下丘脑弓状核腹内侧部分（Arc）的Fos表达。在下丘脑和后脑的其他核团中未观察到Fos表达的变化。Arc中约90%的Fos阳性神经元表达神经肽Y（NPY）信使核糖核酸。这些数据表明，Arc中的NPY神经元可能是介导腹腔注射胃饥饿素诱导的促食欲作用的主要靶点。
