Bai Xiaodong, Hogenhout Saskia A
Department of Entomology, Ohio Agricultural Research and Development Center (OARDC), Ohio State University, Wooster 44691, USA.
FEMS Microbiol Lett. 2002 Apr 23;210(1):7-17. doi: 10.1111/j.1574-6968.2002.tb11153.x.
The mollicute corn stunt spiroplasma (Spiroplasma kunkelii) is a leafhopper-transmitted pathogen of maize. Sequencing of the approximately 1.6-Mb genome of S. kunkelii was initiated to aid understanding the genetic basis of spiroplasma interactions with their plant and leafhopper hosts. In total, 144712 nucleotides of non-redundant, high-quality S. kunkelii genome sequence were obtained. Sequence tags were searched against the Mycoplasmataceae and Bacillus/Clostridium databases. Results showed that, in addition to spiroplasma phage SpV1 DNA insertions, spiroplasma genomes harbor more purine and amino acid biosynthesis, transcription regulation, cell envelope and DNA transport/binding genes than Mycoplasmataceae genomes. This investigation demonstrates that survey sequencing is an efficient procedure for gene discovery and genome characterization. The results of the S. kunkelii sequencing project are available at the Spiroplasma WebPage at http://www.oardc.ohio-state.edu/spiroplasma/genome.htm.
植原体玉米矮化螺原体（Spiroplasma kunkelii）是一种由叶蝉传播的玉米病原体。启动了对约1.6兆碱基的S. kunkelii基因组的测序，以帮助理解螺原体与其植物和叶蝉宿主相互作用的遗传基础。总共获得了144712个非冗余、高质量的S. kunkelii基因组序列核苷酸。将序列标签与支原体科和芽孢杆菌/梭菌数据库进行比对。结果表明，除了螺原体噬菌体SpV1 DNA插入片段外，螺原体基因组中嘌呤和氨基酸生物合成、转录调控、细胞包膜以及DNA转运/结合基因比支原体科基因组更多。这项研究表明，调查测序是一种用于基因发现和基因组特征描述的有效方法。S. kunkelii测序项目的结果可在螺旋体网页（http://www.oardc.ohio-state.edu/spiroplasma/genome.htm）上获取。
