Liu Peng, Li Yuxin, Ye Youyuan, Chen Jiaxin, Li Rong, Zhang Qinyi, Li Yuan, Wang Wen, Meng Qingguo, Ou Jingyu, Yang Zhujun, Sun Wei, Gu Wei
Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Basic Medical School, Hengyang Medical School, Institute of Pathogenic Biology, University of South China, Hengyang, China.
Key Laboratory for Aquatic Crustacean Diseases, College of Marine Science and Engineering, Nanjing Normal University, Nanjing, China.
Front Microbiol. 2022 Nov 2;13:996938. doi: 10.3389/fmicb.2022.996938. eCollection 2022.
, small motile wall-less bacteria, was originally isolated from a rabbit tick and had the ability to infect newborn mice and caused cataracts. In this study, the whole genome and antigen proteins of were comparative analyzed and investigated. Glycolysis, pentose phosphate pathway, arginine metabolism, nucleotide biosynthesis, and citrate fermentation were found in , while trichloroacetic acid, fatty acids metabolism, phospholipid biosynthesis, terpenoid biosynthesis, lactose-specific PTS, and cofactors synthesis were completely absent. The Sec systems of consist of SecA, SecE, SecDF, SecG, SecY, and YidC. Signal peptidase II was identified in , but no signal peptidase I. The relative gene order in is largely conserved. Genome analysis of available species in revealed that they shared only 84 proteins. genome has 381 pseudogenes, accounting for 31.6% of total protein-coding genes. This is the evidence that spiroplasma genome is under an ongoing genome reduction. Immunoproteomics, a new scientific technique combining proteomics and immunological analytical methods, provided the direction of our research on . We identified 49 proteins and 11 proteins (9 proteins in common) in by anti- serum and negative serum, respectively. Forty proteins in were identified in relation to the virulence. All these proteins may play key roles in the pathogeny and can be used in the future for diagnoses and prevention.
支原体，一种小型、可运动、无细胞壁的细菌，最初是从兔蜱中分离出来的，具有感染新生小鼠并导致白内障的能力。在本研究中，对支原体的全基因组和抗原蛋白进行了比较分析和研究。在支原体中发现了糖酵解、磷酸戊糖途径、精氨酸代谢、核苷酸生物合成和柠檬酸盐发酵，而三氯乙酸、脂肪酸代谢、磷脂生物合成、萜类生物合成、乳糖特异性磷酸转移酶系统和辅因子合成则完全缺失。支原体的Sec系统由SecA、SecE、SecDF、SecG、SecY和YidC组成。在支原体中鉴定出了信号肽酶II，但没有信号肽酶I。支原体中的相关基因顺序在很大程度上是保守的。对支原体中现有物种的基因组分析表明，它们仅共享84种蛋白质。支原体基因组有381个假基因，占总蛋白质编码基因的31.6%。这证明支原体基因组正在经历持续的基因组缩减。免疫蛋白质组学，一种将蛋白质组学和免疫分析方法相结合的新科学技术，为我们对支原体的研究提供了方向。我们分别用抗血清和阴性血清在支原体中鉴定出了49种蛋白质和11种蛋白质（其中9种相同）。在支原体中鉴定出了40种与毒力相关的蛋白质。所有这些蛋白质可能在致病过程中起关键作用，未来可用于诊断和预防。
