阴沟肠杆菌以外的肠杆菌属中的染色体AmpC基因，以及ACT-1质粒编码的头孢菌素酶与阿氏肠杆菌的祖先关联。
Chromosomal ampC genes in Enterobacter species other than Enterobacter cloacae, and ancestral association of the ACT-1 plasmid-encoded cephalosporinase to Enterobacter asburiae.
作者信息
Rottman Martin, Benzerara Yahia, Hanau-Berçot Béatrice, Bizet Chantal, Philippon Alain, Arlet Guillaume
机构信息
Service de Bactériologie, Hôpital Tenon, UFR Saint Antoine, Paris, France.
出版信息
FEMS Microbiol Lett. 2002 Apr 23;210(1):87-92. doi: 10.1111/j.1574-6968.2002.tb11164.x.
The amplification and sequence of ampC genes in Enterobacter asburiae, Enterobacter cancerogenus, Enterobacter dissolvens, Enterobacter hormaechei and Enterobacter intermedius bring the number of known cephalosporinase sequences from the genus Enterobacter to seven. Expression in Escherichia coli of the ampC genes from E. asburiae, E. hormaechei and E. intermedius established the functional nature of these genes. ampC from E. asburiae shows 96.5% identity to bla(ACT-1) encoding a plasmid-borne cephalosporinase previously believed to derive from Enterobacter cloacae. The reassignment of ACT-1 ancestry to E. asburiae is confirmed by the 95.5% identity between ampR upstream of bla(ACT-1) and ampR from E. asburiae.
阿氏肠杆菌、致癌肠杆菌、溶解肠杆菌、霍氏肠杆菌和中间肠杆菌中ampC基因的扩增及测序，使得肠杆菌属已知头孢菌素酶序列的数量增至7个。阿氏肠杆菌、霍氏肠杆菌和中间肠杆菌的ampC基因在大肠杆菌中的表达确定了这些基因的功能特性。阿氏肠杆菌的ampC与编码一种先前认为源自阴沟肠杆菌的质粒介导头孢菌素酶的bla(ACT-1)具有96.5%的同一性。bla(ACT-1)上游的ampR与阿氏肠杆菌的ampR之间95.5%的同一性证实了ACT-1的祖先被重新认定为阿氏肠杆菌。
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