Evans Ingrid A C, Jones Cheryl A
Herpesvirus Research Unit, Children's Hospital at Westmead, Westmead, New South Wales 2145, Australia.
J Infect Dis. 2002 Jun 1;185(11):1550-60. doi: 10.1086/340572. Epub 2002 May 17.
Herpes simplex virus (HSV) causes devastating infections in newborns. Maternal immunization is one potential strategy to reduce neonatal HSV disease. Female mice were immunized with an HSV type 2 (HSV-2) replication-defective mutant (HSV-2 dl5-29, which is defective for the genes UL5 and UL29) and then mated. Protection was evaluated in newborn mice after a virulent HSV-2 oral challenge. Heightened neonatal susceptibility was observed to a thymidine kinase-negative HSV-2 strain (HSV-2 186DeltaKpn) that is highly attenuated in adult mice. Maternal immunization with HSV-2 dl5-29 and HSV-2 186DeltaKpn reduced visceral spread of infectious challenge virus in pups after challenge at either 1 day or 1 week of age but did not prevent replication at the site of entry, spread to the central nervous system, or lethal encephalitis. No protection was seen in pups born to mock-immunized mothers or to mothers immunized with a UV-inactivated wild-type HSV-2 strain. Levels of protection correlated with levels of passively transferred maternal HSV-2-specific IgG antibody.
单纯疱疹病毒（HSV）可导致新生儿发生严重感染。母体免疫是降低新生儿HSV疾病的一种潜在策略。用2型单纯疱疹病毒（HSV-2）复制缺陷型突变体（HSV-2 dl5-29，其UL5和UL29基因存在缺陷）对雌性小鼠进行免疫，然后使其交配。在用强毒力HSV-2经口攻击后，对新生小鼠的保护作用进行评估。观察到对胸苷激酶阴性的HSV-2毒株（HSV-2 186DeltaKpn）新生小鼠易感性增强，该毒株在成年小鼠中高度减毒。用HSV-2 dl5-29和HSV-2 186DeltaKpn对母体进行免疫，可降低1日龄或1周龄幼崽在感染性攻击病毒攻击后感染病毒在内脏的扩散，但不能阻止病毒在进入部位复制、扩散至中枢神经系统或致死性脑炎。在假免疫母亲或用紫外线灭活的野生型HSV-2毒株免疫的母亲所生的幼崽中未观察到保护作用。保护水平与被动转移的母体HSV-2特异性IgG抗体水平相关。
