细胞周期蛋白依赖性激酶8在斑马鱼胚胎发育过程中由母体和合子共同表达。

Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development.

作者信息

Brabazon Elaine D, Bree Ronan T, Carton Micheal W, Grealy Maura, Byrnes Lucy

机构信息

Department of Biochemistry and the National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland.

出版信息

Biochim Biophys Acta. 2002 Jun 7;1576(1-2):203-8. doi: 10.1016/s0167-4781(02)00302-0.

DOI:10.1016/s0167-4781(02)00302-0

PMID:12031503

Abstract

Cyclin-dependent kinase 8 (cdk8) regulates transcription by phosphorylating RNA polymerase II and TFIIH. The mechanism of zygotic transcription activation during vertebrate embryonic development is poorly understood. Here we describe the cloning and developmental expression pattern of zebrafish cdk8 mRNA. It is highly conserved, sharing 79% DNA and 95% amino acid sequence identity with human cdk8, thereby indicating an important role for the protein. Northern blotting and whole mount in situ hybridisation revealed expression of zebrafish cdk8 maternally, following the onset of zygotic transcription at the mid-blastula transition (MBT) and throughout embryonic development.

摘要

细胞周期蛋白依赖性激酶8（cdk8）通过磷酸化RNA聚合酶II和TFIIH来调节转录。脊椎动物胚胎发育过程中合子转录激活的机制尚不清楚。在这里，我们描述了斑马鱼cdk8 mRNA的克隆及发育表达模式。它高度保守，与人类cdk8的DNA序列一致性为79%，氨基酸序列一致性为95%，这表明该蛋白具有重要作用。Northern印迹法和整体原位杂交显示，斑马鱼cdk8在母源期、囊胚中期转换（MBT）时合子转录开始后以及整个胚胎发育过程中均有表达。