Palmer Helen, Ohta Mari, Watanabe Masumi, Suzuki Tetsuya
Laboratory of Food Wholesomeness, Department of Life Sciences, Graduate School of Fisheries Science, Hokkaido University, 3-1-1 Minato, Hakodate 041-8611, Japan.
J Photochem Photobiol B. 2002 Jun;67(2):116-29. doi: 10.1016/s1011-1344(02)00271-3.
The effects of ultraviolet radiation (UV-A: 320-400 nm and UV-B: 280-320 nm) and methyl viologen (MV) single or combined exposure, on the cell growth, viability and morphology of two strains of the unicellular flagellate Euglena gracilis, using the Z strain as a plant model and the achlorophyllous mutant SMZ strain as an animal model were investigated. Cell growth was not affected by MV only, whereas UV-A or UV-B single and combined exposure with MV inhibited the cell growth or decreased the viability. The SMZ strain had a higher number of abnormal cells than the Z strain after the third dose of UV-B was delivered simultaneously with MV. The abnormal cell number decreased when E. gracilis SMZ cells were preincubated with 100 microM rutin prior to the UV-B and MV exposure. There were higher abnormal cell numbers with groups exposed to UV rather than MV single exposure. Combined exposure to UV-B and 200 microM MV induced the highest levels of TBARS in both strains, and with the supplementation of rutin these high levels were suppressed. These results suggest that UV-A or UV-B irradiation alone or combined with MV cause considerable oxidative damage in E. gracilis cells, and rutin supplementation may suppress their adverse effects.
研究了紫外线辐射（UV-A：320 - 400纳米和UV-B：280 - 320纳米）和甲基紫精（MV）单独或联合暴露，对两株单细胞鞭毛虫纤细裸藻细胞生长、活力及形态的影响，其中以Z株作为植物模型，无叶绿素的突变体SMZ株作为动物模型。单独的MV对细胞生长没有影响，而UV-A或UV-B单独以及与MV联合暴露均抑制细胞生长或降低活力。当第三次给予UV-B并同时给予MV时，SMZ株的异常细胞数量比Z株更多。在UV-B和MV暴露之前，将纤细裸藻SMZ细胞与100微摩尔芦丁预孵育后，异常细胞数量减少。暴露于紫外线的组比单独暴露于MV的组有更高的异常细胞数量。UV-B与200微摩尔MV联合暴露在两株藻中均诱导了最高水平的丙二醛，而补充芦丁后这些高水平受到抑制。这些结果表明，单独的UV-A或UV-B照射或与MV联合，会在纤细裸藻细胞中造成相当大的氧化损伤，补充芦丁可能会抑制它们的不利影响。
