Danilov R A, Ekelund N G
Department of Applied Science, Mid Sweden University, 871 88 Härnösand, Sweden.
Environ Toxicol. 2001;16(1):78-83. doi: 10.1002/1522-7278(2001)16:1<78::aid-tox90>3.0.co;2-9.
The responses of the growth rate, motility, and cell shape in the green flagellate Euglena gracilis to different concentrations of waste water substances from the pulp and paper industry were tested in a long-term study (7 days). Samples before (uncleaned sample) and after (cleaned sample) the treatment in a cleaning system were studied. The influence of different doses of UV-B radiation on cell shape and motility was also investigated. No statistically significant effects of increasing concentrations of the waste substances both before and after the cleaning process (except inhibition by the undiluted uncleaned effluent) on the growth rate in E. gracilis were observed. Cell shape turned out to be an unreliable physiological parameter for assessing the toxicity at lower concentrations of waste water substances. No significant patterns could be observed in the response of the cell shape to the different concentrations of the waste water substances or to UV-B radiation. Motility has been concluded to be a more sensitive parameter than cell shape. However, no clear patterns were observed in the response of the motility to the different concentrations of the waste water substances studied. Increasing concentrations of the uncleaned sample demonstrated a defense against UV-B radiation, due to the high absorbance in the UV-B range, when effects on motility were examined. We conclude that contrary to the results reported in the literature earlier, cell shape and motility of E. gracilis are not universal physiological parameters for bioassessment at lower concentrations of toxic substances, including metals such as copper and zinc from the pulp and paper industry. The long-duration tests had, in general, higher significance than those of short duration.
在一项长期研究（7天）中，测试了绿藻眼虫（Euglena gracilis）的生长速率、运动性和细胞形态对造纸工业不同浓度废水物质的反应。研究了清洁系统处理前（未清洁样品）和处理后（清洁样品）的样本。还研究了不同剂量的UV-B辐射对细胞形态和运动性的影响。在清洁过程前后，未观察到废水物质浓度增加对纤细裸藻生长速率有统计学上的显著影响（未稀释的未清洁废水有抑制作用除外）。结果表明，细胞形态对于评估较低浓度废水物质的毒性来说是一个不可靠的生理参数。在细胞形态对不同浓度废水物质或UV-B辐射的反应中，未观察到显著规律。已得出结论，运动性是比细胞形态更敏感的参数。然而，在所研究的不同浓度废水物质对运动性的反应中，未观察到明显规律。当研究对运动性的影响时，由于未清洁样品在UV-B范围内的高吸光度，其浓度增加表现出对UV-B辐射的防御作用。我们得出结论，与早期文献报道的结果相反，对于包括造纸工业中的铜和锌等金属在内的较低浓度有毒物质的生物评估，纤细裸藻的细胞形态和运动性并非通用的生理参数。一般来说，长期测试比短期测试具有更高的显著性。
