Inoue Koichi, Wada Megumi, Higuchi Tae, Oshio Shigeru, Umeda Takashi, Yoshimura Yoshihiro, Nakazawa Hiroyuki
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Jun 25;773(2):97-102. doi: 10.1016/s1570-0232(02)00101-0.
The potential risks to human health and reproduction from the xenoestrogen bisphenol A (BPA) have not been well established. This is due in part to the absence of accurate analytical methods to quantify BPA in biological samples. In this study we establish an accurate, sensitive and selective analytical method for the quantification of BPA in human semen. To quantify BPA we compared the techniques of liquid chromatography-mass spectrometry (LC-MS) and enzyme-linked immunosorbent assay (ELISA). In addition we have taken steps to eliminate BPA contamination during sample extraction and preparation. Results show that the ELISA method gives an over-estimate of BPA concentration, which may be due, at least in part, to non-specific interactions with the BPA-antibodies. LC-MS gave much more accurate results and proved to be more sensitive with a detection limit of 0.5 ng ml(-1) compared to 2.0 ng ml(-1) by ELISA.
环境雌激素双酚A（BPA）对人类健康和生殖的潜在风险尚未完全明确。部分原因在于缺乏准确的分析方法来定量生物样品中的BPA。在本研究中，我们建立了一种准确、灵敏且具选择性的分析方法，用于定量人类精液中的BPA。为了定量BPA，我们比较了液相色谱 - 质谱联用（LC - MS）技术和酶联免疫吸附测定（ELISA）。此外，我们已采取措施在样品提取和制备过程中消除BPA污染。结果表明，ELISA方法高估了BPA浓度，这可能至少部分归因于与BPA抗体的非特异性相互作用。LC - MS给出了更准确的结果，并且被证明更灵敏，检测限为0.5 ng/ml，而ELISA的检测限为2.0 ng/ml。
