Lai Xinan, Wang Zhengguo, Wei Li, Wang Lili
Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Chin J Traumatol. 2002 Jun;5(3):176-9.
To explore the relationship between substance P (SP) released from peripheral nerve endings and the expression of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) during wound healing.
Fifty Wistar rats were randomly divided into 2 groups, injury group and capsaicin group. In the injury group, a full-thickness skin wound on the back of the rat was taken. The wound edge and granulation tissues were taken on the 1st, 3rd, 6th, 9th, 12th days after injury, respectively. In the capsaicin group, capsaicin was injected subcutaneously on the back of the rats to destroy the sensory nerve to prevent the secretion of SP, then a wound and sample was made in the same way. Immunohistochemistry and in situ hybridization were employed to detect the expression of SP, EGF/EGFR, and EGF mRNA/EGFR mRNA in the granulation tissues.
In the injury group, immunohistochemical stain of SP and EGF/EGFR was located on the hair follicles and sebaceous glands at the 1st day. And the stain of SP was obvious at the 3rd day in the granulation tissues, then decreased gradually. EGF/EGFR was at low level at the 3rd day, then increased gradually and reached the peak at the 9th day, then declined. In the capsaicin group, the immunohistochemical stain of SP and EGF/EGFR was faint and without obvious change during the wound healing process. The tendency of the EGF mRNA/EGFR mRNA expression was similar to that of EGF/EGFR.
During wound healing, SP may promote the healing process by affecting the expression of EGF/EGFR in the granulation tissues.
探讨伤口愈合过程中外周神经末梢释放的P物质（SP）与表皮生长因子（EGF）及表皮生长因子受体（EGFR）表达之间的关系。
将50只Wistar大鼠随机分为2组，即损伤组和辣椒素组。损伤组在大鼠背部制作全层皮肤伤口，分别于伤后第1、3、6、9、12天取伤口边缘及肉芽组织。辣椒素组于大鼠背部皮下注射辣椒素破坏感觉神经以阻止SP分泌，然后以同样方法制作伤口并取材。采用免疫组织化学和原位杂交法检测肉芽组织中SP、EGF/EGFR及EGF mRNA/EGFR mRNA的表达。
损伤组伤后第1天，SP和EGF/EGFR免疫组织化学染色位于毛囊和皮脂腺。肉芽组织中第3天SP染色明显，随后逐渐减弱。第3天EGF/EGFR处于低水平，随后逐渐升高并于第9天达到峰值，然后下降。辣椒素组中，SP和EGF/EGFR免疫组织化学染色较淡，在伤口愈合过程中无明显变化。EGF mRNA/EGFR mRNA表达趋势与EGF/EGFR相似。
在伤口愈合过程中，SP可能通过影响肉芽组织中EGF/EGFR的表达来促进愈合过程。
