通过旋转坐标系中的核磁共振弛豫研究琼脂糖凝胶中的水。
Water in agarose gels studied by nuclear magnetic resonance relaxation in the rotating frame.
作者信息
Andrasko J
出版信息
Biophys J. 1975 Dec;15(12):1235-43. doi: 10.1016/S0006-3495(75)85896-6.
The dependence of the spin-lattice relaxation time in the rotating frame (T1rho) on radio frequency (RF) field strength and temperature has been studied for agarose gels in order to investigate molecular motion. The results indicate the presence of slow motions with a correlation time of ca. 5-10(-6) s at room temperature. This interaction is responsible for the short spin-spin relaxation times (T2) for water protons in agarose gels and is ascribed to firmly bound water. The fraction of bound water is estimated to about 0.003 for a 7.3% agarose gel. The motion of the more mobile protons in agarose-water systems can not be characterized by single correlation time. This fraction is presumably composed of water in different motional states and some of the agarose hydroxyl protons. Higher mobilities are the most common.
为了研究分子运动，已对琼脂糖凝胶中旋转坐标系下的自旋晶格弛豫时间（T1rho）与射频（RF）场强及温度的依赖关系进行了研究。结果表明，在室温下存在相关时间约为5 - 10^(-6) 秒的慢运动。这种相互作用导致了琼脂糖凝胶中水质子的短自旋 - 自旋弛豫时间（T2），并归因于牢固结合的水。对于7.3%的琼脂糖凝胶，结合水的比例估计约为0.003。琼脂糖 - 水体系中迁移性较强的质子的运动不能用单一相关时间来表征。该部分可能由处于不同运动状态的水和一些琼脂糖羟基质子组成。较高的迁移率最为常见。
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