寡核苷酸在Spheron离子交换衍生物上的分离

Separation of oligonucleotides on ion-exchange derivatives of Spheron.

作者信息

Satava J, Mikes O, Strop P

出版信息

J Chromatogr. 1979 Nov 28;180(1):31-7. doi: 10.1016/s0021-9673(00)80171-5.

PMID:120376

Abstract

Diphenylamine-formic acid hydrolyzate of Bacillus subtilis DNA (a mixture of pyrimidine oligodeoxyribonucleotides) was chromatographed using an ionic strength gradient on seven ion exchangers: five DEAE-Spherons 300 of various capacities, two types of BD-Spheron 300 and DEAE-Sephadex A-25. The suitability of ion-exchange derivatives of Spheron for the chromatography of oligonucleotides is discussed. The separation of mono- to undeca-deoxyribonucleotides was achieved.

摘要

枯草芽孢杆菌DNA的二苯胺 - 甲酸水解产物（嘧啶寡聚脱氧核糖核苷酸混合物）在七种离子交换剂上使用离子强度梯度进行色谱分析：五种不同容量的DEAE - Spheron 300、两种类型的BD - Spheron 300和DEAE - 葡聚糖A - 25。讨论了Spheron离子交换衍生物用于寡核苷酸色谱分析的适用性。实现了单至十一脱氧核糖核苷酸的分离。