Schott H
Nucleic Acids Symp Ser. 1980(7):203-13.
A simple alternative to complicated chemical synthesis of oligonucleotides is presented. Readily available DNA is partially hydrolyzed to preparative amounts of oligonucleotides using various methods. Chromatographically, pure defined pyrimidine and purine nucleotides, and/or mixtures of sequence isomers, are isolated on preparative scale from the various mixtures of oligonucleotides.
本文提出了一种替代复杂寡核苷酸化学合成的简单方法。使用各种方法将现成的DNA部分水解以制备一定量的寡核苷酸。通过色谱法,从各种寡核苷酸混合物中制备规模地分离出纯的特定嘧啶和嘌呤核苷酸,以及/或者序列异构体混合物。