Howard C R, Zuckerman A J
J Med Virol. 1979;4(4):303-14. doi: 10.1002/jmv.1890040408.
A solid-phase radioimmunoassay has been used successfully for detecting hepatitis B e antigen in fractionated hepatitis B virus-containing serum. Ammonium sulphate precipitation followed by gel filtration through a column of Sepharose CL-6B resulted in two fractions of antigen-containing material with molecular weights of 220,000 and 130,000. The smaller of these two fractions was found to possess an average isoelectric point of 4.9 and consisted of two major polypeptide species with estimated molecular weights of 66,000 and 17,000 respectively. Affinity chromatography on Blue Sepharose showed that e antigen was not retained under conditions which bound serum albumin. These results are discussed in relation to the immunopathogenesis of hepatitis B.
一种固相放射免疫测定法已成功用于检测分级分离的含乙型肝炎病毒血清中的乙型肝炎e抗原。硫酸铵沉淀后通过Sepharose CL - 6B柱进行凝胶过滤，得到了两部分含抗原物质，分子量分别为220,000和130,000。发现这两部分中较小的一部分平均等电点为4.9，由两种主要多肽组成，估计分子量分别为66,000和17,000。在蓝色琼脂糖凝胶上进行亲和层析表明，在结合血清白蛋白的条件下，e抗原不被保留。结合乙型肝炎的免疫发病机制对这些结果进行了讨论。
