Yamada E, Ohori H, Ishida N
J Med Virol. 1979;4(1):33-42. doi: 10.1002/jmv.1890040105.
Physicochemical studies of hepatitis B e antigen (HBeAg) revealed a clear cut difference between e1 and e2 antigen. The e1 antigen was found to have a MW of Ca 150,000 and a pI of 6.4-7.2, whereas both the MW and pI of the e2 antigen were heterogeneous depending upon the source of serum. Sera obtained from asymptomatic carriers were characterized by low titers of HBs antigen, HBc antigen and DNA polymerase and contained e2 antigen of larger molecular weight (200,000-300,000) with a narrow distribution range and a pI of 4.8 to 5.2 (type 1). On the other hand, the sera from patients in a hemodialysis unit who were HBs antigen carriers and had high titers of HBs antigen, HBc antigen and DNA polymerase contained e2 antigen of heterogeneous distribution in MW (from 300,000 to 70,000) and pI (type 2 and 3). The e2 antigen obtained from the higher MW type 3 serum had lower isoelectric points (pI 4.5 to 5.2) as was the case with e2 antigen obtained from asymptomatic carriers whereas relatively wide range of isoelectric points (pI 5.1 to 8.2) was found with the lower molecular weight e2 antigen.
乙型肝炎e抗原（HBeAg）的物理化学研究揭示了e1抗原和e2抗原之间存在明显差异。发现e1抗原的分子量约为150,000，等电点为6.4 - 7.2，而e2抗原的分子量和等电点则因血清来源而异。从无症状携带者获得的血清具有低滴度的乙肝表面抗原（HBs抗原）、乙肝核心抗原（HBc抗原）和DNA聚合酶，并且含有分子量较大（200,000 - 300,000）、分布范围窄且等电点为4.8至5.2的e2抗原（1型）。另一方面，来自血液透析单位的乙肝表面抗原携带者且具有高滴度乙肝表面抗原、乙肝核心抗原和DNA聚合酶的患者血清中，e2抗原的分子量（从300,000至70,000）和等电点呈异质分布（2型和3型）。从较高分子量的3型血清中获得的e2抗原的等电点较低（4.5至5.2），这与从无症状携带者获得的e2抗原情况相同，而较低分子量的e2抗原的等电点范围相对较宽（5.1至8.2）。
