Kertcher J A, Guilarte T R, Chen M F, Rider A A, McIntyre P A
J Nucl Med. 1979 May;20(5):419-23.
A radiometric microbiologic assay has been developed for the determination of niacin in biologic fluids. Lactobacillus plantarum produced 14CO2 from L-[U-14C] hr malic acid in quantities proportional to the amount of niacin present. The assay is specific for the biologically active forms of niacin in humans. Thirty normal hemolysates were analyzed and the values ranged from 13.0 to 17.8 micrograms niacin/ml RBC (mean = 15.27 +/- 1.33 s.d.). Good recovery and reproducibility studies were obtained with this assay. On thirty blood samples, correlation was excellent between the radiometric and the conventional turbidimetric assays.
已开发出一种放射性微生物测定法，用于测定生物体液中的烟酸。植物乳杆菌从L-[U-¹⁴C]苹果酸产生¹⁴CO₂，其产量与存在的烟酸量成正比。该测定法对人体中具有生物活性形式的烟酸具有特异性。分析了30份正常溶血产物，烟酸含量范围为13.0至17.8微克/毫升红细胞（平均值=15.27±1.33标准差）。该测定法具有良好的回收率和重现性研究。在30份血样上，放射性测定法与传统比浊法之间的相关性极佳。
