Winter J, Kandler O
Arch Microbiol. 1977 Feb 4;112(1):99-102. doi: 10.1007/BF00446660.
No fumarase activity could be found in whole cells or in cell-free crude extracts from Leuconostoc mesenteroides or Lactobacillus curvatus. The degradation of L-malate-4-14C by these organisms yielded more than 95% of the label as 14CO2. It is therefore recommended that these organisms, rather than Lactobacillus plantarum, should be used in the determination of isotope distribution in L-malate-14C, since L. plantarum exhibits a significant fumarase activity and thus randomizes malate prior to the decarboxylation of this substance by the malolactic enzyme.
在嗜温明串珠菌或弯曲乳杆菌的全细胞或无细胞粗提物中均未发现富马酸酶活性。这些微生物对L-苹果酸-4-¹⁴C的降解产生的¹⁴CO₂中，超过95%带有放射性标记。因此，建议在测定L-¹⁴C苹果酸的同位素分布时，应使用这些微生物而非植物乳杆菌，因为植物乳杆菌表现出显著的富马酸酶活性，从而在该物质被苹果酸乳酸酶脱羧之前使苹果酸发生随机化。
