Pavie-Fischer J, Kourilsky F M, Picard F, Banzet P, Puissant A
Clin Exp Immunol. 1975 Sep;21(3):430-41.
The in vitro cytotoxicity of lymphocytes from forty-seven melanoma patients and thirteen healthy subjects for cultured melanoma cells was studied using a 51Cr release assay. Two different melanoma cell lines were used as target cells: one cultured in suspension (SK Mel1) and one tissue culture line growing as a monolayer (NK I1). The lymphocytes from most healthy subjects were found to be cytotoxic for these cultured cells, with individual variations. These repeatable cytotoxic reactions could not be explained on the grounds of previous isoimmunization. The lymphocytes from melanoma patients were also cytotoxic for the melanoma cell lines, but the highest degree of cytotoxicity was found in patients with primitive and localized tumours, and not in patients with metastases.
采用51Cr释放试验研究了47例黑色素瘤患者及13名健康受试者的淋巴细胞对培养的黑色素瘤细胞的体外细胞毒性。使用两种不同的黑色素瘤细胞系作为靶细胞：一种在悬浮液中培养（SK Mel1），另一种作为单层生长的组织培养系（NK I1）。发现大多数健康受试者的淋巴细胞对这些培养细胞具有细胞毒性，存在个体差异。这些可重复的细胞毒性反应无法用先前的同种免疫来解释。黑色素瘤患者的淋巴细胞对黑色素瘤细胞系也具有细胞毒性，但细胞毒性最高的是原发性局限性肿瘤患者，而非转移性患者。
