Byrne J A, Soloski M, Holowczak J A
Cancer Immunol Immunother. 1983;16(2):81-7. doi: 10.1007/BF00199236.
Cell-mediated immune responses in DBA/2 mice bearing melanoma tumors (TB-mice) were measured and compared to similar responses in mice without tumors (C-mice). Splenic lymphocytes from TB-mice had a reduced capacity to respond to both B and T-cell mitogens, but TB-mice responded to infection with vaccinia virus by developing a virus-specific cytotoxic T-cell response equal to that measured with splenic effectors prepared from virus-infected C-mice. NK-cell activity, as measured by the in vitro lysis of YAC-1 targets by splenic effectors, was significantly depressed in TB-mice but, after infection of the animals with vaccinia virus, was restored to levels equal to that measured with splenic effectors prepared from C-mice. Doses of vaccinia virus, strain WR which elicited vaccinia-virus-specific cytotoxic T cells or stimulated NK-cell activity, failed to elicit or stimulate cytotoxic effectors specific for S91-melanoma tumor cells.
对携带黑色素瘤肿瘤的DBA/2小鼠（TB小鼠）的细胞介导免疫反应进行了测量，并与无肿瘤小鼠（C小鼠）的类似反应进行了比较。TB小鼠的脾淋巴细胞对B细胞和T细胞有丝分裂原的反应能力降低，但TB小鼠对痘苗病毒感染的反应是产生与从病毒感染的C小鼠制备的脾效应细胞所测得的相等的病毒特异性细胞毒性T细胞反应。通过脾效应细胞对YAC-1靶标的体外裂解来测量的NK细胞活性在TB小鼠中显著降低，但在用痘苗病毒感染动物后，恢复到与从C小鼠制备的脾效应细胞所测得的水平相等。引发痘苗病毒特异性细胞毒性T细胞或刺激NK细胞活性的WR株痘苗病毒剂量，未能引发或刺激对S91黑色素瘤肿瘤细胞具有特异性的细胞毒性效应细胞。
