He Yu-Qing, Sun Mei, Zhu Ying-Guo, Zhang Li-Da
Department of Zoology, Hong Kong University, Hong Kong, China.
Yi Chuan Xue Bao. 2002 May;29(5):461-6.
This study analyzed the characteristics of reverse transcriptase sequences of copia-like retrotransposons in Amaranthus via PCR amplification and sequence approaches. The results are as follows: (1) The reverse transcriptase sequences of copia-like retrotransposons were detected in all 30 Amaranthus accessions studied, indicating that this class of retrotransposons is ubiquitous in Amaranthus; (2) Twenty-eight reverse transcriptases in A. quitensis were cloned and sequenced, showing extensive heterogeneity in base deletion mutation and stop codon mutation; (3) Cluster and alignment analyses of the 28 sequences and other published sequences of the reverse transcriptase from other species (such as rice, tomato and Drosophila) showed that the copia-like retrotransposons in A. quitensis have a close relationship with those in dicot and monocot plants. The evolution of copia-like retrotransposons in A. tricolor, which might be of the same origin as the copia and 1731 in Drosophila, was discussed.
本研究通过PCR扩增和测序方法分析了苋属植物中类copia逆转录转座子的逆转录酶序列特征。结果如下：（1）在所研究的30份苋属材料中均检测到类copia逆转录转座子的逆转录酶序列，表明这类逆转录转座子在苋属中普遍存在；（2）克隆并测序了昆诺阿藜中的28个逆转录酶，其在碱基缺失突变和终止密码子突变方面表现出广泛的异质性；（3）对这28个序列与其他物种（如水稻、番茄和果蝇）已发表的逆转录酶序列进行聚类和比对分析，结果表明昆诺阿藜中的类copia逆转录转座子与双子叶植物和单子叶植物中的类copia逆转录转座子关系密切。文中还讨论了三色苋中类copia逆转录转座子的进化，其可能与果蝇中的copia和1731起源相同。
