Tseng L, Tseng J K, Escarcena L, Gurpide E
Endocrinology. 1975 Dec;97(6):1481-5. doi: 10.1210/endo-97-6-1481.
The interconversion of labeled estrone (E1) and estradiol (E2) in rabbit uterus was studied in vivo and in vitro, in order to evaluate the influence of incubation conditions upon the preferred direction of the reversible conversion of E2 to E1. In a typical experiment, one uterine horn was perfused intraluminally in situ with a mixture of [3H]E2 and [14C]E1, while the other horn was dissected and used for in vitro perfusions with the same tracers, both intraluminally and on tissue slices. The ratios of rate constants corresponding to the oxidative and reductive reactions were found to be similar under the three perfusion conditions, as calculated from the isotopic data. The rate constant of conversion of E1 to E2 was 10-20 times larger than the rate constant of the opposite reaction. Equal E2 nuclear saturation levels were obtained by perfusion of the uterine tissue with excess [3H]E2 in situ and in vitro after slicing.
为了评估孵育条件对雌二醇（E2）向雌酮（E1）可逆转化的优先方向的影响，对家兔子宫中标记的E1和E2的相互转化进行了体内和体外研究。在一个典型实验中，一个子宫角在原位经腔内灌注[3H]E2和[14C]E1的混合物，而另一个子宫角被解剖并用于相同示踪剂的体外灌注，包括腔内灌注和组织切片灌注。根据同位素数据计算，在三种灌注条件下，对应于氧化和还原反应的速率常数之比相似。E1向E2转化的速率常数比相反反应的速率常数大10 - 20倍。在切片后，通过在体内和体外对子宫组织灌注过量的[3H]E2，获得了相等的E2核饱和水平。
