线粒体和细胞质核糖体RNA与线粒体和核DNA的杂交。

Hybridization of mitochondrial and cytoplasmic ribosomal RNA with mitochondrial and nuclear DNA.

作者信息

Dierich A, Wintzerith M, Mandel P

出版信息

Experientia. 1975 Oct 15;31(10):1151-2. doi: 10.1007/BF02326763.

PMID:1204726

Abstract

Hybridization assays of rat liver mitochondrial and cytoplasmic rRNAs with in vitro labelled mitochondrial and nuclear DNA were performed in liquid medium. Sensitivity towards S1 enzyme and Tms of the RNA-DNA hybrids were studied. Our results are in favour of a distinct genetic origin of the two types of cellular rRNAs.

摘要

在液体培养基中对大鼠肝脏线粒体和细胞质核糖体RNA（rRNA）与体外标记的线粒体和核DNA进行杂交试验。研究了RNA-DNA杂交体对S1酶的敏感性和熔解温度（Tm）。我们的结果支持两种类型的细胞rRNA具有不同的遗传起源。