遗传复杂性对核糖核酸 - 脱氧核糖核酸杂交时间进程的影响。
The effect of genetic complexity on the time-course of ribonucleic acid-deoxyribonucleic acid hybridization.
作者信息
Bishop J O
出版信息
Biochem J. 1969 Aug;113(5):805-11. doi: 10.1042/bj1130805.
Abstract
- The rate of RNA-DNA hybridization was studied under conditions of RNA excess, with RNA synthesized in vitro. The initial rate of the reaction was proportional to the initial RNA concentration. Throughout the observed course of the reaction there was a linear relationship between the reciprocal of the amount of RNA hybridized/mug. of DNA and the reciprocal of time. The slope of the reciprocal plot was inversely proportional to the initial RNA concentration. 2. A comparison was made of the hybridization of DNA from Escherichia coli and from bacteriophages T4 and lambda with homologous RNA. The initial rate of hybridization was inversely proportional to the genetic complexity of the hybridizing system. The slope of the reciprocal-time plot was directly proportional to genetic complexity. These results are interpreted to indicate that the rate of hybridization reflects the mean concentration of the various unique RNA species in a preparation.
摘要
- 在RNA过量的条件下，对体外合成的RNA进行RNA-DNA杂交速率的研究。反应的初始速率与初始RNA浓度成正比。在整个观察到的反应过程中，每微克DNA杂交的RNA量的倒数与时间的倒数之间存在线性关系。倒数图的斜率与初始RNA浓度成反比。2. 对来自大肠杆菌、噬菌体T4和λ的DNA与同源RNA的杂交进行了比较。杂交的初始速率与杂交系统的遗传复杂性成反比。倒数时间图的斜率与遗传复杂性成正比。这些结果被解释为表明杂交速率反映了制剂中各种独特RNA种类的平均浓度。
相似文献
1
The effect of genetic complexity on the time-course of ribonucleic acid-deoxyribonucleic acid hybridization.遗传复杂性对核糖核酸 - 脱氧核糖核酸杂交时间进程的影响。
Biochem J. 1969 Aug;113(5):805-11. doi: 10.1042/bj1130805.
2
Transcription of bacteriophage T4 deoxyribonucleic acid in vitro.噬菌体T4脱氧核糖核酸的体外转录
Biochem J. 1969 Nov;115(3):353-61. doi: 10.1042/bj1150353.
3
Methods for the analysis of deoxyribonucleic acid-ribonucleic acid hybridization data.脱氧核糖核酸-核糖核酸杂交数据分析方法。
Biochem J. 1969 Nov;115(3):361-70. doi: 10.1042/bj1150361.
4
Molecular hybridization of ribonucleic acid with a large excess of deoxyribonucleic acid.核糖核酸与大量脱氧核糖核酸的分子杂交
Biochem J. 1972 Jan;126(1):171-85. doi: 10.1042/bj1260171.
5
Control of gene function in bacteriophage T4. I. Ribonucleic acid and deoxyribonucleic acid metabolism in T4rII-infected lambda-lysogenic hosts.噬菌体T4中基因功能的控制。I. T4rII感染的λ溶原宿主中的核糖核酸和脱氧核糖核酸代谢
J Virol. 1969 Nov;4(5):742-52. doi: 10.1128/JVI.4.5.742-752.1969.
6
Examination of an equilibrium interpretation of deoxyribonucleic acid-ribonucleic acid hybridization data.对脱氧核糖核酸-核糖核酸杂交数据的平衡解释的检验。
Biochem J. 1970 Jan;116(2):223-8. doi: 10.1042/bj1160223.
7
RNA-primed DNA synthesis in vitro.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1560-4. doi: 10.1073/pnas.69.6.1560.
8
Ribonucleic acid synthesis during microcyst formation in Myxococcus xanthus: characterization by deoxyribonucleic acid-ribonucleic acid hybridization.黄色黏球菌微囊形成过程中的核糖核酸合成：通过脱氧核糖核酸-核糖核酸杂交进行表征
J Bacteriol. 1970 Oct;104(1):275-82. doi: 10.1128/jb.104.1.275-282.1970.
9
Characterization of rapidly labelled ribonucleic acid in Escherichia coli by deoxyribonucleic acid-ribonucleic acid hybridization.通过脱氧核糖核酸-核糖核酸杂交对大肠杆菌中快速标记的核糖核酸进行表征
Biochem J. 1968 Nov;110(2):251-63. doi: 10.1042/bj1100251.
10
Genetic transcription directed by the b2 region of lambda bacteriophage.由λ噬菌体b2区域指导的基因转录。
Proc Natl Acad Sci U S A. 1968 Dec;61(4):1320-7. doi: 10.1073/pnas.61.4.1320.
引用本文的文献
1
The genes for cytoplasmic ribosomal ribonucleic Acid in higher plants.高等植物中细胞质核糖体核糖核酸的基因。
Plant Physiol. 1973 Apr;51(4):677-84. doi: 10.1104/pp.51.4.677.
2
Transcriptional origin of Euglena chloroplast tRNAs.眼虫叶绿体tRNA的转录起源
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1984-8. doi: 10.1073/pnas.73.6.1984.
3
Molecular hybridization between rat liver deoxyribonucleic acid and complementary ribonucleic acid.大鼠肝脏脱氧核糖核酸与互补核糖核酸之间的分子杂交
Biochem J. 1970 Nov;120(2):225-35. doi: 10.1042/bj1200225.
4
Examination of an equilibrium interpretation of deoxyribonucleic acid-ribonucleic acid hybridization data.对脱氧核糖核酸-核糖核酸杂交数据的平衡解释的检验。
Biochem J. 1970 Jan;116(2):223-8. doi: 10.1042/bj1160223.
5
The number of sex-factors per chromosome in Escherichia coli.大肠杆菌中每条染色体的性因子数量。
Biochem J. 1971 Jan;121(1):93-103. doi: 10.1042/bj1210093.
6
A method for measuring the content of a specific messenger ribonucleic acid in Escherichia coli.一种测量大肠杆菌中特定信使核糖核酸含量的方法。
Biochem J. 1971 Jan;121(1):105-8. doi: 10.1042/bj1210105.
7
Transcription of bacteriophage T4 deoxyribonucleic acid in vitro.噬菌体T4脱氧核糖核酸的体外转录
Biochem J. 1969 Nov;115(3):353-61. doi: 10.1042/bj1150353.
8
Ribonucleic acid-deoxyribonucleic acid hybridization as a second-order reaction.核糖核酸-脱氧核糖核酸杂交作为二级反应
Biochem J. 1973 Nov;135(3):573-6. doi: 10.1042/bj1350573.
9
Molecular hybridization of ribonucleic acid with a large excess of deoxyribonucleic acid.核糖核酸与大量脱氧核糖核酸的分子杂交
Biochem J. 1972 Jan;126(1):171-85. doi: 10.1042/bj1260171.
10
The chromosomal localisation of human satellite DNA I.人类卫星DNA I的染色体定位。
Chromosoma. 1974;49(2):161-71. doi: 10.1007/BF00348888.
本文引用的文献
1
Thermal renaturation of deoxyribonucleic acids.脱氧核糖核酸的热复性
J Mol Biol. 1961 Oct;3:585-94. doi: 10.1016/s0022-2836(61)80023-5.
2
A fractionating column for analysis of nucleic acids.用于核酸分析的分馏柱。
Anal Biochem. 1960 Jun;1:66-77. doi: 10.1016/0003-2697(60)90020-8.
3
4
FORMATION AND PROPERTIES OF RNA-DNA COMPLEXES.RNA-DNA复合物的形成与特性
J Mol Biol. 1964 Jul;9:125-42. doi: 10.1016/s0022-2836(64)80095-4.
5
6
Thermal conversion of nonpriming deoxyribonucleic acid to primer.非引发脱氧核糖核酸向引物的热转化
J Biol Chem. 1959 Oct;234:2733-4.
7
Sequence complementarity of T2-DNA and T2-specific RNA.T2-DNA与T2特异性RNA的序列互补性。
Proc Natl Acad Sci U S A. 1961 Feb 15;47(2):137-63. doi: 10.1073/pnas.47.2.137.
8
The enzymatic synthesis of RNA: complementary interaction with DNA.RNA的酶促合成：与DNA的互补相互作用。
Proc Natl Acad Sci U S A. 1961 Sep 15;47(9):1405-15. doi: 10.1073/pnas.47.9.1405.
9
Kinetics of renaturation of DNA.DNA复性动力学
J Mol Biol. 1968 Feb 14;31(3):349-70. doi: 10.1016/0022-2836(68)90414-2.
10
Hybridization between rat liver DNA and complementary RNA.
J Mol Biol. 1969 Feb 28;40(1):117-36. doi: 10.1016/0022-2836(69)90300-3.
Zotero 插件