枯草芽孢杆菌营养缺陷型菌株在营养有限的培养基中的生长与孢子形成。

Growth and sporulation of auxotrophs of Bacillus subtilis in a medium with limited nutrients.

作者信息

Nishihara T

出版信息

Microbiol Immunol. 1979;23(8):727-34. doi: 10.1111/j.1348-0421.1979.tb00515.x.

DOI:10.1111/j.1348-0421.1979.tb00515.x

PMID:120486

Abstract

Growth and sporulation were examined for 30 auxotrophs of Bacillus subtilis in a chemically defined medium with suboptimal amounts of nutrients. All strains except for some adenine-requiring mutants could not overtake sporulation stage II when amino acids, vitamins, or bases were limited, whereas they sporulated fairly well without limitation. Abnormal structures, a cell with thickened cell wall and a cell with several refractile bodies, were found in some strains after the vegetative growth stopped.

摘要

在营养成分含量次优的化学限定培养基中，对枯草芽孢杆菌的30种营养缺陷型菌株的生长和孢子形成进行了检测。当氨基酸、维生素或碱基受到限制时，除了一些需要腺嘌呤的突变体之外，所有菌株均无法进入孢子形成的第二阶段，而在没有限制的情况下它们能较好地形成孢子。在营养生长停止后，在一些菌株中发现了异常结构，即细胞壁增厚的细胞和含有几个折光体的细胞。