Runx1表达标记了妊娠中期小鼠胚胎中的长期重建造血干细胞。

Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo.

作者信息

North Trista E, de Bruijn Marella F T R, Stacy Terryl, Talebian Laleh, Lind Evan, Robin Catherine, Binder Michael, Dzierzak Elaine, Speck Nancy A

机构信息

Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA.

出版信息

Immunity. 2002 May;16(5):661-72. doi: 10.1016/s1074-7613(02)00296-0.

DOI:10.1016/s1074-7613(02)00296-0

PMID:12049718

Abstract

Hematopoietic stem cells (HSCs) are first found in the aorta-gonad-mesonephros region and vitelline and umbilical arteries of the midgestation mouse embryo. Runx1 (AML1), the DNA binding subunit of a core binding factor, is required for the emergence and/or subsequent function of HSCs. We show that all HSCs in the embryo express Runx1. Furthermore, HSCs in Runx1(+/-) embryos are heterogeneous and include CD45(+) cells, endothelial cells, and mesenchymal cells. Comparison with wild-type embryos showed that the distribution of HSCs among these various cell populations is sensitive to Runx1 dosage. These data provide the first morphological description of embryonic HSCs and contribute new insight into their cellular origin.

摘要

造血干细胞（HSCs）最早在妊娠中期小鼠胚胎的主动脉-性腺-中肾区域以及卵黄囊和脐动脉中被发现。核心结合因子的DNA结合亚基Runx1（AML1）是造血干细胞出现和/或后续功能所必需的。我们发现胚胎中的所有造血干细胞都表达Runx1。此外，Runx1(+/-)胚胎中的造血干细胞是异质性的，包括CD45(+)细胞、内皮细胞和间充质细胞。与野生型胚胎的比较表明，造血干细胞在这些不同细胞群体中的分布对Runx1剂量敏感。这些数据首次对胚胎造血干细胞进行了形态学描述，并为其细胞起源提供了新的见解。