GP3: GenePix post-processing program for automated analysis of raw microarray data.

作者信息

Fielden M R, Halgren R G, Dere E, Zacharewski T R

机构信息

Department of Biochemistry & Molecular Biology, National Food Safety & Toxicology Center, Institute for Environmental Toxicology, Michigan State University, East Lansing, MI 48824, USA.

出版信息

Bioinformatics. 2002 May;18(5):771-3. doi: 10.1093/bioinformatics/18.5.771.

DOI:10.1093/bioinformatics/18.5.771

PMID:12050078

Abstract

UNLABELLED

Here we describe an automated and customizable program to correct, filter and normalize raw microarray data captured using GenePix, a commonly used microarray image analysis application. Files can be processed individually or in batch mode for increased throughput. User defined inputs specify the stringency of data filtering and the method and conditions of normalization. The output includes gene summaries for replicate spots and descriptive statistics for each experiment. The source code (Perl) can also be adapted to handle raw data output from other image analysis applications.