Logan N A, Forsyth G, Lebbe L, Goris J, Heyndrickx M, Balcaen A, Verhelst A, Falsen E, Ljungh A, Hansson H B, De Vos P
Int J Syst Evol Microbiol. 2002 May;52(Pt 3):953-966. doi: 10.1099/00207713-52-3-953.
Thirty-three clinical, dairy and industrial isolates of aerobic endospore-forming bacteria which were unreactive in routine identification tests were characterized genotypically by using amplified rDNA restriction analysis (ARDRA), 16S rDNA sequencing and DNA-DNA reassociation, and phenotypically by using fatty acid methyl ester (FAME) analysis, SDS-PAGE of whole-cell proteins, API Biotype 100 assimilation tests and 16 other routine phenotypic tests. Three isolates were identified as strains of Bacillus badius, 12 as Brevibacillus agri, including 3 strains associated with an outbreak of waterborne illness, 4 as Brevibacillus centrosporus and 2 as Brevibacillus parabrevis; 12 strains contaminating an antibiotic production plant were recognized as members of a new species, for which the name Brevibacillus invocatus is proposed, with the type strain LMG 18962T (= B2156T = CIP 106911T = NCIMB 13772T).
33株需氧产芽孢细菌的临床、乳制品和工业分离株在常规鉴定试验中无反应，通过扩增rDNA限制性分析（ARDRA）、16S rDNA测序和DNA-DNA重联进行基因型特征分析，并通过脂肪酸甲酯（FAME）分析、全细胞蛋白质的SDS-PAGE、API Biotype 100同化试验和其他16项常规表型试验进行表型特征分析。3株分离株被鉴定为短芽孢杆菌菌株，12株为农业短芽孢杆菌，其中3株与一次水源性疾病暴发有关，4株为中心芽孢短芽孢杆菌，2株为短短芽孢杆菌；12株污染抗生素生产厂的菌株被确认为一个新物种的成员，为此提出了“召唤短芽孢杆菌”这一名称，模式菌株为LMG 18962T（=B2156T=CIP 106911T=NCIMB 13772T）。
