关于牛初乳胰蛋白酶抑制剂的碳水化合物组成

On the carbohydrate composition of bovine colostrum trypsin inhibitor.

作者信息

Tschesche H, Klauser R, Cechová D, Jonáková V

出版信息

Hoppe Seylers Z Physiol Chem. 1975 Nov;356(11):1759-64. doi: 10.1515/bchm2.1975.356.2.1759.

DOI:10.1515/bchm2.1975.356.2.1759

PMID:1205451

Abstract

The trypsin inhibitor from bovine colostrum has been separated into several forms by CM-Sephadex and DEAE-cellulose chromatography. These forms differ in the amount and composition of the carbohydrate they contain, which has been quantitated for four components by gas-liquid chromatography and standrad colorimetric procedures. The monosaccharides fucose, mannose, galactose, galactosamine, glucosamine and sialic acids have been determined. A microheterogeneity was establish ed in the carbohydrate moiety, which amounts to about 40% of the total molecular weight (Mr 11 000 - 14 000) of bovine colostrum inhibitor.

摘要

通过CM-葡聚糖凝胶和二乙氨基乙基纤维素色谱法，已将牛初乳中的胰蛋白酶抑制剂分离成几种形式。这些形式所含碳水化合物的数量和组成不同，已通过气液色谱法和标准比色法对四种成分进行了定量。已测定了单糖岩藻糖、甘露糖、半乳糖、半乳糖胺、葡萄糖胺和唾液酸。在碳水化合物部分确定了微观不均一性，其含量约占牛初乳抑制剂总分子量（Mr 11 000 - 14 000）的40%。