Nitta Yumiko, Tanaka Hideo, Masuda Yuji, Hoshi Masaharu
International Radiation Information Center, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi 1-2-3, Minami-Ku, Hiroshima 734-8553, Japan.
J Radiat Res. 2002 Mar;43(1):65-75. doi: 10.1269/jrr.43.65.
It is well known that the yield of DNA recovered form tissues preserved in formalin is inversely proportional to the stored duration. How is the quality? We tested the quality of DNA from archival tissues of atomic-bomb survivors stored in formalin for decades with the parameters of gene amplification efficiency by a polymerase chain reaction. All of the DNA extracted from the tissues preserved in formalin for 30 years amplified the 54- and 61-base pairs of the DNA fragments successfully. The direct sequencing of the PCR products confirmed the accurate amplification of the target sequence. A further trial to amplify the longer sequence of 111 base pairs succeeded in 20% of the samples tested. From these results, we propose a new utility of archival samples for the analysis of single nucleotide sequence polymorphism of genes, no matter how long the samples have been preserved in formalin.
众所周知，从福尔马林固定保存的组织中回收的DNA产量与保存时间成反比。那么质量如何呢？我们通过聚合酶链反应，以基因扩增效率为参数，测试了储存数十年的原子弹幸存者档案组织中的DNA质量。从福尔马林固定保存30年的组织中提取的所有DNA均成功扩增出了54和61个碱基对的DNA片段。PCR产物的直接测序证实了靶序列的准确扩增。在20%的测试样本中，进一步扩增111个碱基对更长序列的试验取得了成功。基于这些结果，我们提出了档案样本在基因单核苷酸序列多态性分析中的新用途，无论样本在福尔马林中保存了多长时间。
