Noori Sadat, Monabati Ahmad, Ghaderi Abbasali
Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Med Sci. 2012 Jun;37(2):126-33.
Carcinomas of esophagus, mostly squamous cell carcinomas, occur throughout the world. There are a number of suspected genetic or environmental etiologies. Human papilloma virus (HPV) is said to be a major etiology in areas with high incidence of esophageal carcinoma, while it is hardly detectable in low incidence regions. This study was designed to evaluate the prevalence of HPV in esophageal squamous cell carcinoma (ESCC) cases diagnosed in Pathology Department, Medical School, Shiraz University of Medical Sciences.
DNA material for PCR amplification of HPV genome was extracted from formalin-fixed paraffin-embedded tissue blocks of 92 cases of ESCC, diagnosed during 20 years from 1982 to 2002. Polymerase chain reaction was performed for amplification and detection of common HPV and type specific HPV-16 and HPV-18 genomic sequences in the presence of positive control (HPV-18 and HPV positive biopsies of uterine exocervix) and additional internal controls i.e. beta-globin and cytotoxic T lymphocyte antigen 4 (CTLA4).
Good amplification of positive control and internal controls was observed. However, no amplification of HPV genome was observed.
There is no association between HPV infection and the development of esophageal squamous cell carcinoma in the cases evaluated.
食管癌，多数为鳞状细胞癌，在全球范围内均有发生。存在多种疑似的遗传或环境病因。人乳头瘤病毒（HPV）据说是食管癌高发地区的主要病因，而在低发地区则很难检测到。本研究旨在评估设拉子医科大学医学院病理科诊断的食管鳞状细胞癌（ESCC）病例中HPV的感染率。
从1982年至2002年20年间诊断的92例ESCC的福尔马林固定石蜡包埋组织块中提取用于HPV基因组PCR扩增的DNA材料。在阳性对照（HPV-18和子宫颈HPV阳性活检组织）以及额外的内对照即β-珠蛋白和细胞毒性T淋巴细胞抗原4（CTLA4）存在的情况下，进行聚合酶链反应以扩增和检测常见HPV以及特定类型的HPV-16和HPV-18基因组序列。
观察到阳性对照和内对照扩增良好。然而，未观察到HPV基因组的扩增。
在所评估的病例中，HPV感染与食管鳞状细胞癌的发生之间没有关联。
